Source: University of Arizona
New research untangles some of the mysteries of a protein called EVL (pronounced “evil”), which may reduce the ability of a common kind of breast cancer to spread to other parts of the body.
About 80 percent of all breast cancers are estrogen receptor-positive, in which estrogen fuels cancer growth. The new discovery could have implications for developing more precise treatments for this estrogen receptor-positive, or ER-positive, breast cancer.
As reported in Nature Communications, researchers used epidemiological data to compare breast cancer patients taking hormone replacement therapy at the time of diagnosis with those who were not. They analyzed genetic data to identify EVL as an important regulator of cancer cells’ ability to spread, and then conducted follow-up experiments in breast cancer cells.
Estrogen-filled ‘gas tanks’
ER-positive breast tumors are studded with estrogen receptors, which are like gas tanks that can be filled with estrogen, fueling cancer’s growth. Currently, patients receive treatment through surgery and chemotherapy, typically followed with anti-estrogenic drugs such as tamoxifen, which reduces cancer recurrence by cutting off breast cancer cells’ fuel supply.
The estrogen story is not “black and white,” however. Cancer is most dangerous when cells break free from the original tumor and travel to other areas of the body, a process called metastasis. But tumor cells can replicate without metastasizing, remaining contained in their original site. Although estrogen does increase the risk for the initiation of breast cancer and fuel its growth, it does not seem to promote metastasis.
“In breast cancer, estrogen is connected to the growth of the tumor, but it also seems to suppress the ability of cancer to spread,” says Marco Padilla-Rodriguez, a recent graduate of the University of Arizona College of Medicine-Tucson’s Graduate Program in Molecular Medicine.
“The growth of the tumor and the tumor’s ability to spread aren’t always linked. You might have a large tumor, but it stays contained. You might have a small tumor that spreads throughout the body,” Padilla-Rodriguez says.
Researchers in the lab of Ghassan Mouneimne, assistant professor of cellular and molecular medicine, analyzed genetic datasets to identify EVL as a factor in taming estrogen’s cancerous effects. They found that estrogen enhances the production of EVL, which seems to keep cancer cells contained to the original tumor site. As estrogen levels fall, so do levels of EVL, freeing cancer cells to invade neighboring tissues—the first step in metastasis. EVL’s role in regulating a cell’s actin cytoskeleton could be the key to its ability to suppress cell movement.
“Cells have skeletons, just like we have a skeleton,” Padilla-Rodriguez explains. “Actin is one type of skeleton. Unlike our skeleton, actin can be remodeled, like Lego pieces being shaped into different structures.”
Depending on how a cell rearranges its actin cytoskeleton, it might be more likely to stay in one place, adhering to adjacent cells, or it could have the ability to migrate, crawling away from other cells like a microscopic caterpillar.
“Generally, cells like to cluster together,” Padilla-Rodriguez says. “When we treat them with anti-estrogenic drugs, actin allows the cell to break away and pull itself forward.”
One of the next steps is to learn how to leverage EVL’s interaction with estrogen to develop combination treatments for patients with ER-positive tumors. While anti-estrogenic drugs rein in tumor growth, they also might indirectly reduce EVL levels, accelerating the remaining cancer cells’ invasion of neighboring tissues.
“With tamoxifen, you’re inhibiting the brakes,” Mouneimne says “Now we want to go after the gas pedal to halt the cancer from progressing. Then we will be inhibiting both growth and invasion.”
Mouneimne is working with Tech Launch Arizona to commercialize a method to measure EVL levels in ER-positive breast tumors, with the hope that someday such a diagnostic could help expand personalized medicine options for breast cancer patients.
Additional coauthors are from the University of Arizona; the Dana-Farber Cancer Institute; the Howard Hughes Medical Institute; and Institut de Recherche sur les Maladies Virales et Hépatiques in Strasbourg, France.
The National Cancer Institute, an NCI diversity supplemental grant, a University of Arizona Cancer Center Support Grant, and a Science Foundation Arizona Bisgrove Scholars Postdoctoral Fellowship funded the work.
Barbara Jacoby is an award winning blogger that has contributed her writings to multiple online publications that have touched readers worldwide.